Keywords
Cover cropping
Sclerotium rolfsii
Sclerotinia sclerotiorum
Macrophomina phaseolina
How to Cite
Abstract
Brassica biofumigant cover crops are being increasingly considered in vegetable crop rotations as part of an integrated disease management strategy and simply as a cover cropping choice. Nine biofumigant varieties were assessed to see if they could be grown year-round in the Lockyer Valley South East Queensland region, for yield, days to incorporation and glucosinolate concentrations, as well as efficacy against 3 soilborne pathogens; Sclerotinia sclerotiorum, Sclerotium rolfsii and Macrophomina phaseolina. The fastest growing brassica biofumigant was BQ Mulch which reached 25% flowering in 36 and 59 days from planting to incorporation with a summer and winter planting respectively. Nemcon and Nemclear took the longest to incorporation when planted in summer, 101 days and failed to flower, while Caliente, Tillage Radish and Biofum reached 25% flowering and incorporation in 98 days when planted in winter. BQ Mulch produced the least amount of biomass, 30.93 t/ha fresh weight and 2.92 t/ha dry weight with a summer planting. Biofum producing the greatest amount of biomass, 185.76 t/ha fresh weight and 17.34 t/ha dry weight with a summer and winter planting respectively. Most varieties produced more total glucosinolates during summer compared to winter. Caliente produced the highest levels of Total GSLs with 53.47 µmol/g DW in summer compared to 23.78 µmol/g DW in winter. This was reflected in their efficacy against the soilborne pathogens. Caliente and Mustclean were more efficacious at controlling Macrophomina and Sclerotinia in summer compared to winter while all varieties were more efficacious at controlling Sclerotinia with a summer planting compared to a winter planting.
References
Thorup-Kristensen K, Magid J, Jensen LS. Catch crops and green manures as biological tools in nitrogen management in temperate zones. Advances in agronomy. 2003; 79(79): 227-302. https://doi.org/10.1016/S0065-2113(02)79005-6
Kator L, Hosea ZY, Oche OD. Sclerotium rolfsii: causative organism of southern blight, stem rot, white mold and sclerotia rot disease. Annals Biol Res. 2015; 6(11): 78-89.
Alexander BJR, Stewart A. Survival of sclerotia of Sclerotinia and Sclerotium spp in New Zealand horticultural soil. Soil Biology and Biochemistry. 1994; 26(10): 1323-9. https://doi.org/10.1016/0038-0717(94)90213-5
Adams P, Ayers W. Ecology of Sclerotinia species. Phytopathology. 1979; 69(8): 896-9. https://doi.org/10.1094/Phyto-69-896
Watanabe T. Survivability of Macrophomina phaseoli (Maubl.) Ashby in Naturally-infested Soils and Longevity of the Sclerotia Formed in vitro. Annals of the Phytopathological Society of Japan. 1973; 39(4): 333-7. https://doi.org/10.3186/jjphytopath.39.333
Gupta GK, Sharma SK, Ramteke R. Biology, epidemiology and management of the pathogenic fungus Macrophomina phaseolina (Tassi) Goid with special reference to charcoal rot of soybean (Glycine max (L.) Merrill). Journal of Phytopathology. 2012; 160(4): 167-80. https://doi.org/10.1111/j.1439-0434.2012.01884.x
Larkin RP, Griffin TS, Honeycutt CW. Rotation and cover crop effects on soilborne potato diseases, tuber yield, and soil microbial communities. Plant Disease. 2010; 94(12): 1491-502. https://doi.org/10.1094/PDIS-03-10-0172
Sharma A, Saha TN, Arora A, Shah R, Nain L. Efficient Microorganism Compost Benefits Plant Growth and Improves Soil Health in Calendula and Marigold. Horticultural Plant Journal. 2017; 3(2): 67-72. https://doi.org/10.1016/j.hpj.2017.07.003
Kirkegaard J, Gardner P, Desmarchelier J, Angus J, editors. Biofumigation: using Brassica species to control pests and diseases in horticulture and agriculture. Proceedings 9th Australian Research Assembly on Brassicas; 1993; Agricultural Research Institute, Wagga.
Kirkegaard J. Biofumigation for Plant Disease Control - from the Fundamentals to the Farming System. Disease Control in Crops: Biological and Environmentally Friendly Approaches: Wiley-Blackwell; 2009. p. 172-95. https://doi.org/10.1002/9781444312157.ch9
Milford G, Evans E. Factors causing variation in glucosinolates in oilseed rape. Outlook on Agriculture. 1991; 20(1): 31-7. https://doi.org/10.1177/003072709102000107
Kirkegaard JA, Sarwar M. Biofumigation potential of brassicas: I. Variation in glucosinolate profiles of diverse field-grown brassicas. Plant and Soil. 1998; 201(1): 71-89. https://doi.org/10.1023/A: 1004364713152
Charron CS, Saxton AM, Sams CE. Relationship of climate and genotype to seasonal variation in the glucosinolate-myrosinase system. I. Glucosinolate content in ten cultivars of Brassica oleracea grown in fall and spring seasons. Journal of the Science of Food and Agriculture. 2005; 85(4): 671-81. https://doi.org/10.1002/jsfa.1880
Sarwar M, Kirkegaard JA, Wong PTW, Desmarchelier JM. Biofumigation potential of brassicas III. In vitro toxicity of isothiocyanates to soil-borne fungal pathogens. Plant and Soil. 1998; 201(1): 103-12. https://doi.org/10.1023/A: 1004381129991
Warmington R, Clarkson JP. Volatiles from biofumigant plants have a direct effect on carpogenic germination of sclerotia and mycelial growth of Sclerotinia sclerotiorum. Plant and Soil. 2016; 401(1): 213-29. https://doi.org/10.1007/s11104-015-2742-8
Smith BJ, Kirkegaard JA. In vitro inhibition of soil microorganisms by 2-phenylethyl isothiocyanate. Plant Pathology. 2002; 51(5): 585-93. https://doi.org/10.1046/j.1365-3059.2002.00744.x
West L, Tsui I, Haas G. Single column approach for the liquid chromatographic separation of polar and non-polar glucosinolates from broccoli sprouts and seeds. Journal of Chromatography A. 2002; 966(1-2): 227-32. https://doi.org/10.1016/S0021-9673(02)00734-3
Williams DJ, Critchley C, Pun S, Nottingham S, O'Hare TJ. Epithiospecifier protein activity in broccoli: The link between terminal alkenyl glucosinolates and sulphoraphane nitrile. Phytochemistry. 2008; 69(16): 2765-73. https://doi.org/10.1016/j.phytochem.2008.09.018
Duff JD, van Sprang Celia, O'Halloran Julie, Hall Zara. Guide to Brassica Biofumigant Cover Crops Managing soilborne diseases in vegetable production systems. State of Queensland: Department of Agriculture and Fisheries; 2020. https://www.publications.qld.gov.au/dataset/brassica-biofumigant-cover-crops/resource/a3694e67-0b1c-4972-a290-b8606a7c7360
Rosa E, Heaney R, Fenwick G, Portas C. Glucosinolates in crop plants. 1997.
Sarwar M, Kirkegaard JA. Biofumigation potential of brassicas II. Effect of environment and ontogeny on glucosinolate production and implications for screening. Plant and Soil. 1998; 201(1): 91-101. https://doi.org/10.1023/A: 1004333230899
Justen VL, Fritz VA. Temperature-induced glucosinolate accumulation is associated with expression of BrMYB transcription factors. HortScience. 2013; 48(1): 47-52. https://doi.org/10.21273/HORTSCI.48.1.47
Zhang H, Schonhof I, Krumbein A, Gutezeit B, Li L, Stützel H, et al. Water supply and growing season influence glucosinolate concentration and composition in turnip root (Brassica rapa ssp. rapifera L.). J Plant Nutr Soil Sci. 2008; 171(2): 255-65. https://doi.org/10.1002/jpln.200700079
Charron CS, Sams CE. Glucosinolate content and myrosinase activity in rapid-cycling Brassica oleracea grown in a controlled environment. Journal of the American Society for Horticultural Science. 2004; 129(3): 321-30. https://doi.org/10.21273/JASHS.129.3.0321
del Carmen Martínez-Ballesta M, Moreno D, Carvajal M. The physiological importance of glucosinolates on plant response to abiotic stress in Brassica. International journal of molecular sciences. 2013; 14(6): 11607-25. https://doi.org/10.3390/ijms140611607
Björkman M, Klingen I, Birch AN, Bones AM, Bruce TJ, Johansen TJ, et al. Phytochemicals of Brassicaceae in plant protection and human health-Influences of climate, environment and agronomic practice. Phytochemistry. 2011; 72(7): 538-56. https://doi.org/10.1016/j.phytochem.2011.01.014
Mathur V, Tytgat TO, de Graaf RM, Kalia V, Reddy AS, Vet LE, et al. Dealing with double trouble: consequences of single and double herbivory in Brassica juncea. Chemoecology. 2013; 23(2): 71-82. https://doi.org/10.1007/s00049-012-0120-z
Mewis I, Appel HM, Hom A, Raina R, Schultz JC. Major signaling pathways modulate Arabidopsis glucosinolate accumulation and response to both phloem-feeding and chewing insects. Plant Physiology. 2005; 138(2): 1149-62. https://doi.org/10.1104/pp.104.053389
Schlatter D, Kinkel L, Thomashow L, Weller D, Paulitz T. Disease Suppressive Soils: New Insights from the Soil Microbiome. Phytopathology. 2017; 107(11): 1284-97. https://doi.org/10.1094/PHYTO-03-17-0111-RVW
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