Keywords
Static magnetic field
Iron oxide nanoparticle
Dracocephalum polychaetum
Enzymatic antioxidant defense system
How to Cite
Abstract
This study was conducted to investigate the effect of magnetic iron oxide nanoparticles (MNP) and static magnetic field (SMF) on the activity of antioxidant enzymes in the cell suspension culture of Dracocephalum polychaetum (Lamiaceae family). The treatment procedure was done by cultivating the cells either with 100 ppm MNP, SMFs, or simultaneous exposure to both MNP and SMFs. The SMF at 30 mT was uniformly applied to the cells either for 3 or 4 days with 3 hours per day or 5 hours per day intervals, respectively. The highest activity of polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), catalase (CAT), malondialdehyde (MDA) content, and electrical conductivity (EC) were observed under the elicitation of the cells with simultaneous exposure to both MNP and SMFs, but the highest amount of FRAP value was observed under the elicitation of the sample with the MNP treatment. Also, the results of this study showed that the greatest activity of peroxidase (POX) was observed under SMF and MNP treatments. In general, SMF and MNP treatments caused various changes in cell structure and metabolism by inducing oxidative stress and having a direct effect on the membrane. The cell activated its enzymatic antioxidant defense system in response to these treatments, which caused changes in its activity and amount compared to the control cell.
References
Maffei ME. Magnetic field effects on plant growth, development, and evolution. Front Plant Sci. 2014; 5: Article 445. https://doi.org/10.3389/fpls.2014.00445
Belyavskaya NA. Biological effects due to weak magnetic field on plants. Adv Space Res. 2004; 34: 1566-74. https://doi.org/10.1016/j.asr.2004.01.021
Taghizadeh M, Nasibi F, Manouchehri Kalantari K, Mohseni-Moghadam M. Modification of phytochemical production and antioxidant activity of Dracocephalum kotschyi cells by exposure to static magnetic field and magnetite nanoparticles. Plant Cell Tissue Organ Cult. 2021; 147: 365-77. https://doi.org/10.1007/s11240-021-02129-9
Hafeez MB, Zahra N, Ahmad N, Shi Z, Raza A, Wang X, Li J. Growth, physiological, biochemical and molecular changes in plants induced by magnetic fields: A review. Plant Biol. 2023; 25: 8-23. https://doi.org/10.1111/plb.13459
Yang B, Cheng L, Liu Z, Zhao Y, Xu A. Impact of SMFs on microorganisms, plants, and animals. In: Zhang X, Biological Effects of Static Magnetic Fields, Singapore: Springer; 2023, p. 187-237. https://doi.org/10.1007/978-981-19-8869-1_7
Jalali M, Ghanati F, Modarres-Sanavi AM, Khoshgoftarmanesh AH. Physiological effects of repeated foliar application of magnetite nanoparticles on maize plants. J Agron Crop Sci. 2017; 203: 593-602. https://doi.org/10.1111/jac.12208
Feng Y, Kreslavski VD, Shmarev AN, Ivanov AA, Zharmukhamedov SK, Kosobryukhov A, et al. Effects of iron oxide nanoparticles (Fe3O4) on growth, photosynthesis, antioxidant activity and distribution of mineral elements in wheat (Triticum aestivum) plants. Plants. 2022; 11(14): 1894. https://doi.org/10.3390/plants11141894
Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962; 15: 473-97. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Taghizadeh M, Bozorgzadeh F, Ghorbani M. Designing magnetic field sensor based on tapered photonic crystal fibre assisted by a ferrofluid. Sci Rep. 2021; 11: Article number: 14325. https://doi.org/10.1038/s41598-021-93568-z
Heath RL, Packer L. Photoperoxidation in isolated chloroplasts. Arch Biochem Biophys. 1968; 125: 189-98. https://doi.org/10.1016/0003-9861(68)90654-1
Oyaizu M. Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr Diet. 1986; 44: 307-15. https://doi.org/10.5264/eiyogakuzashi.44.307
Shokrollahi S, Ghanati F, Sajedi RH, Sharifi M. Possible role of iron containing proteins in physiological responses of soybean to static magnetic field. J Plant Physiol. 2018; 226: 163-71. https://doi.org/10.1016/j.jplph.2018.04.018
Pandya SR, Singh M. Dispersion and optical activities of newly synthesized magnetic nanoparticles with organic acids and dendrimers in DMSO studied with UV/vis spectrophotometry. J Mol Liq. 2015; 211: 146-56. https://doi.org/10.1016/j.molliq.2015.06.068
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72: 248-54. https://doi.org/10.1016/0003-2697(76)90527-3
Abdolmaleki P, Ghanati F, Sahebjamei H, Sarvestani AS. Peroxidase activity, lignification and promotion of cell death in tobacco cells exposed to static magnetic field. Environmentalist. 2007; 27: 435-40. https://doi.org/10.1007/s10669-007-9080-1
Sahebjamei H, Abdolmaleki P, Ghanati F. Effects of magnetic field on the antioxidant enzyme activities of suspension-cultured tobacco cells. Bioelectromagnetics. 2007; 28: 42-7. https://doi.org/10.1002/bem.20262
Cakmak I, Horst WJ. Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant. 1991; 83: 463-8. https://doi.org/10.1034/j.1399-3054.1991.830320.x
Dahajipour Heidarabadi M, Ghanati F, Fujiwara T. Interaction between boron and aluminum and their effects on phenolic metabolism of Linum usitatissimum L. roots. Plant Physiol Biochem. 2011; 49: 1377-83. https://doi.org/10.1016/j.plaphy.2011.09.008
Amrhein N, Gödeke K-H, Gerhardt J. The estimation of phenylalanine ammonia-lyase (PAL)-activity in intact cells of higher plant tissue. Planta. 1976; 131: 33-40. https://doi.org/10.1007/BF00387342
Haghighat N, Abdolmaleki P, Ghanati F, Behmanesh M, Payez A. Modification of catalase and MAPK in Vicia faba cultivated in soil with high natural radioactivity and treated with a static magnetic field. J Plant Physiol. 2014; 171: 99-103. https://doi.org/10.1016/j.jplph.2013.10.019
Kamble SN, Satdive RK, Manwatkar SN, Salunkhe C, Itteera J, Singh K, et al. Influence of magnetic field on the growth, development and rhizome yield of turmeric (Curcuma longa L.). Plant Cell Tissue Organ Cult. 2022; 150: 555-61. https://doi.org/10.1007/s11240-022-02304-6
Ghanati F, Abdolmaleki P, Vaezzadeh M, Rajabbeigi E, Yazdani M. Application of magnetic field and iron in order to change medicinal products of Ocimum basilicum. Environmentalist. 2007; 27: 429-34. https://doi.org/10.1007/s10669-007-9079-7
Batcioglu K, Ozturk I, Atalay S, Dogan D, Bayri N, Demirtas H. Investigation of time dependent magnetic field effects on superoxide dismutase and catalase activity: an in vitro study. J Biol Phys Chem. 2002; 2: 108–12. https://doi.org/10.4024/34020208.jbpc.02.03
Moradbeygi H, Jamei R, Heidari R, Darvishzadeh R. Investigating the enzymatic and non-enzymatic antioxidant defense by applying iron oxide nanoparticles in Dracocephalum moldavica L. plant under salinity stress. Sci Hortic. 2020; 272: 109537. https://doi.org/10.1016/j.scienta.2020.109537
Hassanpour H, Hassanpour S. Promoting impact of electromagnetic field on antioxidant system and performance of vascular tissues in Physalis alkekengi. Russ J Plant Physiol. 2021; 68: 545-51. https://doi.org/10.1134/S1021443721030079
Nasiri M, Hassanpour H, Sorahinobar M, Niknam V. Impact of static magnetic field on the callogenesis, phytochemical production and antioxidant enzymes in Anthemis gilanica. Russ J Plant Physiol. 2022; 69: 77. https://doi.org/10.1134/S1021443722040124
Saletnik B, Saletnik A, Słysz E, Zaguła G, Bajcar M, Puchalska-Sarna A, et al. The static magnetic field regulates the structure, biochemical activity, and gene expression of plants. Molecules. 2022; 27: 5823. https://doi.org/10.3390/molecules27185823
Jamshidi M, Ghanati F, Rezaei A, Bemani E. Change of antioxidant enzymes activity of hazel (Corylus avellana L.) cells by AgNPs. Cytotechnology. 2016; 68: 525-30. https://doi.org/10.1007/s10616-014-9808-y
Regoli F, Gorbi S, Machella N, Tedesco S, Benedetti M, Bocchetti R, et al. Pro-oxidant effects of extremely low frequency electromagnetic fields in the land snail Helix aspersa. Free Radic Biol Med. 2005; 39: 1620-8. https://doi.org/10.1016/j.freeradbiomed.2005.08.004
Li H, Fong R, Woo M, Ahmed H, Seo D-H, Malik R, et al. Toward high-energy Mn-based disordered-rocksalt Li-ion cathodes. Joule. 2022; 6: 53-91. https://doi.org/10.1016/j.joule.2021.11.005
Rout GR, Sahoo S. Role of iron in plant growth and metabolism. Rev Agric Sci. 2015; 3: 1-24. https://doi.org/10.7831/ras.3.1
Dat J, Vandenabeele S, Vranova E, Van Montagu M, Inze* D, Van Breusegem F. Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci. 2000; 57: 779-95. https://doi.org/10.1007/s000180050041
Chen Z, Yin J-J, Zhou Y-T, Zhang Y, Song L, Song M, et al. Dual enzyme-like activities of iron oxide nanoparticles and their implication for diminishing cytotoxicity. ACS Nano. 2012; 6: 4001-12. https://doi.org/10.1021/nn300291r
Balk J, Pilon M. Ancient and essential: the assembly of iron–sulfur clusters in plants. Trends Plant Sci. 2011; 16: 218-26. https://doi.org/10.1016/j.tplants.2010.12.006
Aladjadjiyan A. Influence of stationary magnetic field on lentil seeds. Int Agrophys. 2010; 24: 321–4.
Zhang X, Yarema K, Xu A, Zhang X, Yarema K, Xu A. Impact of static magnetic field (SMF) on microorganisms, plants and animals. In: Zhang X, Yarema K, Xu A, Eds., Biological Effects of Static Magnetic Fields, Singapore: Springer; 2017, p. 133-72. https://doi.org/10.1007/978-981-10-3579-1_5
Fang Y, Wang L, Xin Z, Zhao L, An X, Hu Q. Effect of foliar application of zinc, selenium, and iron fertilizers on nutrients concentration and yield of rice grain in China. J Agric Food Chem. 2008; 56: 2079-84. https://doi.org/10.1021/jf800150z
Potters G, Horemans N, Jansen MAK. The cellular redox state in plant stress biology – A charging concept. J Plant Physiol Biochem. 2010; 48: 292-300. https://doi.org/10.1016/j.plaphy.2009.12.007
Ghanati F, Ishka MR. Improvement of antioxidant system and decrease of lignin by nickel treatment in tea plant. J Plant Nutr. 2006; 29: 1649-61. https://doi.org/10.1080/01904160600851536
Ali A, Alqurainy F. Activities of antioxidants in plants under environmental stress. In: Motohashi N, Ed., The lutein prevention and treatment for diseases. India: Transworld Research Network; 2006, pp. 187–256.
Gupta D, Palma J, Corpas F. Redox State as a Central Regulator of Plant-Cell Stress Responses. Cham: Springer International Publishing; 2016. https://doi.org/10.1007/978-3-319-44081-1
Ren H-X, Liu L, Liu C, He S-Y, Huang J, Li J-L, et al. Physiological investigation of magnetic iron oxide nanoparticles towards Chinese mung bean. J Biomed Nanotechnol. 2011; 7: 677-84. https://doi.org/10.1166/jbn.2011.1338
Li J, Chang PR, Huang J, Wang Y, Yuan H, Ren H. Physiological effects of magnetic iron oxide nanoparticles towards watermelon. J Nanosci Nanotechnol. 2013; 13: 5561–7. https://doi.org/10.1166/jnn.2013.7533
Mhamdi A, Queval G, Chaouch S, Vanderauwera S, Van Breusegem F, Noctor G. Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models. J Exp Bot. 2010; 61: 4197-220. https://doi.org/10.1093/jxb/erq282
Çelik Ö, Büyükuslu N, Atak Ç, Rzakoulieva A. Effects of magnetic field on activity of superoxide dismutase and catalase in Glycine max (L.) Merr. roots. Pol J Environ Stud. 2009; 18(2): 175-82.
Polovinkina EO, Kal’yasova EA, Sinitsina Yu V., Veselov AP. Effect of weak pulse magnetic fields on lipid peroxidation and activities of antioxidant complex components in pea chloroplasts. Russ J Plant Physiol. 2011; 58: 1069-73. https://doi.org/10.1134/S102144371106015X
Hu L, Li H, Pang H, Fu J. Responses of antioxidant gene, protein and enzymes to salinity stress in two genotypes of perennial ryegrass (Lolium perenne) differing in salt tolerance. J Plant Physiol. 2012; 169: 146-56. https://doi.org/10.1016/j.jplph.2011.08.020
Payez A, Ghanati F, Behmanesh M, Abdolmaleki P, Hajnorouzi A, Rajabbeigi E. Increase of seed germination, growth and membrane integrity of wheat seedlings by exposure to static and a 10-KHz electromagnetic field. Electromagn Biol Med. 2013; 32: 417-29. https://doi.org/10.3109/15368378.2012.735625
Sinha S, Saxena R. Effect of iron on lipid peroxidation, and enzymatic and non-enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L. Chemosphere. 2006; 62: 1340-50. https://doi.org/10.1016/j.chemosphere.2005.07.030
Du W, Sun Y, Ji R, Zhu J, Wu J, Guo H. TiO2 and ZnO nanoparticles negatively affect wheat growth and soil enzyme activities in agricultural soil. J Environ Monitor. 2011; 13: 822-8. https://doi.org/10.1039/c0em00611d
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